Isihlungi se-Fluorescence siyingxenye eyinhloko esetshenziswa kumathuluzi e-biomedical nawesayensi yempilo.Umsebenzi wayo oyinhloko ukuhlukanisa nokukhetha isici se-wavelength spectrum kusukela ekukhanyeni okuvusa amadlingozi kanye ne-fluorescence ekhishwayo yento ohlelweni lokuhlola nokuhlaziya lwe-biomedical fluorescence.Izihlungi ze-fluorescence zivamise ukubonakala ngokujula kwe-cut-off kanye ne-autofluorescence ephansi.Ngokuvamile, izihlungi eziningi zingaxhunywa ndawonye ukuze zenze isihlungi se-fluorescence, esinokusebenza kwezindleko eziphezulu.
Isihlungi se-fluorescence sisetshenziswa ensimbini ye-PCR yesikhathi sangempela ye-fluorescence .esetshenziswa kakhulu kubhayoloji yamangqamuzana nokutholwa kokuphepha kokudla kanye nokuqapha ubhubhane lwezempilo yomphakathi nezinye izimboni.
Isihlungi se-fluorescence sisetshenziselwa ukuthola izinhlobo ezihlukahlukene zamadayi nama-probes, isibonelo:
I-FAM/SYBR Okuluhlaza/ Okuluhlaza/HEX/TET/Cy3/JOE/ROX/Cy3.5/Texas Red,Cy5/LC Red640,Cy5.5 njll
Inqubo | (I-IAD Hard Coating) | ||
Ubude begagasi | Ex(nm) | Em(nm) | Isiphambano |
| 470-30 | 525-20 | >6 |
| 523-20 | 564-20 | >6 |
| 543-20 | 584-20 | >6 |
| 571-20 | 612-20 | >6 |
| 628-35 | 692-45 | >6 |
Ukuvimba | OD>6@200~900nm noma @200~1200nm | ||
Umthambeka(nm) | 50 %~ OD5 <10nm | ||
Isiphambano | OD>6 | ||
Usayizi(mm) | Φ4mm, Φ12mm,Φ12.7mm,Φ25.4mm njll. |